mouse anti human il7r monoclonal antibody (R&D Systems)
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mouse anti human il7r monoclonal antibody
![(A) Schematics of the <t>GFP-IL7R</t> splicing reporter, wherein GFP is only expressed by exclusion of IL7R exon 6. (B) Schematics of approach comparing the effects of mutagenesis of functional cis -acting splicing elements to steric blocking of the corresponding elements with ASOs using the GFP-IL7R reporter. The blue dotted lines expand the exon 6 sequence (gray box), and the relevant cis -acting elements are color-coded: enhancer ESE2 (green), silencers ESS2 and ESS3 (red), and 5’ss (yellow). Mutations to ESE2, ESS3 and the 5’SS are shown underneath each element: Δ ESE2 and Δ ESS3 represent mutations to ESE2 and ESS3, respectively, and 5’Cons and 5’Mut represent consensus and crippling mutations to the 5’ss, respectively. The sequence of ASOs targeting these cis -elements (IL7R-001 – IL7R-005) is shown above the exon sequence: IL7R-001 blocks the 5’ss of exon 6, IL7R-002 blocks ESE2, IL7R-003 blocks ESS2 and ESS3, IL7R-004 blocks the sequence in between ESS2 and ESS3, and IL7R-005 blocks ESS3. (C, E) Percentage (%) of exon 6 exclusion (mean ± S.D.) in transcripts from the reporter in HeLa cells stably expressing wild-type or mutant versions of the reporter ( C ), or cells stably expressing the wild-type reporter and transfected with control (ASO-Ctrl) or experimental (IL7R-001 – IL7R-005) morpholino ASOs ( E ) are shown under the gel images. The top band in the gels represents the exon 6 included product whereas the lower band represents the exon 6 excluded product. (D, F) Mean fluorescence intensity (MFI) of GFP expression in the cells from panel C stably expressing the different versions of the reporter ( D ), or in cells from panel E transfected with ASOs ( F ). The left panels show representative GFP MFI histograms for selected mutants (Wild-type, 5’Cons and 5’Mut) ( D ) and selected ASOs (ASO-Ctrl, IL7R-001 and IL7R-005) ( F ), with quantification shown on the right as Log2(Fold-Change) [Log2(FC)] relative to wild-type for all mutants ( D ) and relative to control for all ASOs ( F ). (***) p < 0.001 .](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_29/10__1101_slash_2022__02__22__481529/10__1101_slash_2022__02__22__481529___F1.large.jpg)
Mouse Anti Human Il7r Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+il7r+monoclonal+antibody/Human+IL-7R+alpha%2FCD127+Antibody/bio_rxiv__2022__02__22__481529-213-25-30
Average 94 stars, based on 29 article reviews
Mouse Anti Human Il7r Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+il7r+monoclonal+antibody/Human+IL-7R+alpha%2FCD127+Antibody/bio_rxiv__2022__02__22__481529-213-25-30
Average 94 stars, based on 29 article reviews
mouse anti human il7r monoclonal antibody - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells"
Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells
Journal: bioRxiv
doi: 10.1101/2022.02.22.481529
Figure Legend Snippet: (A) Schematics of the GFP-IL7R splicing reporter, wherein GFP is only expressed by exclusion of IL7R exon 6. (B) Schematics of approach comparing the effects of mutagenesis of functional cis -acting splicing elements to steric blocking of the corresponding elements with ASOs using the GFP-IL7R reporter. The blue dotted lines expand the exon 6 sequence (gray box), and the relevant cis -acting elements are color-coded: enhancer ESE2 (green), silencers ESS2 and ESS3 (red), and 5’ss (yellow). Mutations to ESE2, ESS3 and the 5’SS are shown underneath each element: Δ ESE2 and Δ ESS3 represent mutations to ESE2 and ESS3, respectively, and 5’Cons and 5’Mut represent consensus and crippling mutations to the 5’ss, respectively. The sequence of ASOs targeting these cis -elements (IL7R-001 – IL7R-005) is shown above the exon sequence: IL7R-001 blocks the 5’ss of exon 6, IL7R-002 blocks ESE2, IL7R-003 blocks ESS2 and ESS3, IL7R-004 blocks the sequence in between ESS2 and ESS3, and IL7R-005 blocks ESS3. (C, E) Percentage (%) of exon 6 exclusion (mean ± S.D.) in transcripts from the reporter in HeLa cells stably expressing wild-type or mutant versions of the reporter ( C ), or cells stably expressing the wild-type reporter and transfected with control (ASO-Ctrl) or experimental (IL7R-001 – IL7R-005) morpholino ASOs ( E ) are shown under the gel images. The top band in the gels represents the exon 6 included product whereas the lower band represents the exon 6 excluded product. (D, F) Mean fluorescence intensity (MFI) of GFP expression in the cells from panel C stably expressing the different versions of the reporter ( D ), or in cells from panel E transfected with ASOs ( F ). The left panels show representative GFP MFI histograms for selected mutants (Wild-type, 5’Cons and 5’Mut) ( D ) and selected ASOs (ASO-Ctrl, IL7R-001 and IL7R-005) ( F ), with quantification shown on the right as Log2(Fold-Change) [Log2(FC)] relative to wild-type for all mutants ( D ) and relative to control for all ASOs ( F ). (***) p < 0.001 .
Techniques Used: Mutagenesis, Functional Assay, Blocking Assay, Sequencing, Stable Transfection, Expressing, Transfection, Control, Fluorescence
Figure Legend Snippet: (A) GFP expression in the wild-type reporter cell line transfected with increasing concentrations of control (ASO-Ctrl) or experimental (IL7R-001, IL7R-004, IL7R-005 and IL7R-006) morpholino ASOs. Overlapped representative histograms are shown on the left for each ASO at 0 μM (light gray), 1 μM (blue), 5 μM (green) and 10 μM (dark gray), with quantification of MFI plotted on the right as dose-response curves of Log2(FC) relative to 0 µM for ASO-Ctrl (black), IL7R-001 (blue), IL7R-004 (green), IL7R-005 (red) and IL7R-006 (yellow). (B-C) Percentage (%) of exon 6 exclusion in transcripts from the GFP-IL7R reporter ( B ) or the endogenous IL7R gene ( C ) Representative gel images for each ASO are shown at the top with quantification of percent exon 6 exclusion (mean ± S.D.) plotted at the bottom as a function of ASO concentration (color-coded as in A ). (*) p < 0.05 , (**) p < 0.01 , and (***) p < 0.001 .
Techniques Used: Expressing, Transfection, Control, Concentration Assay
Figure Legend Snippet: (A) Representative gel image of exon 6 splicing in the endogenous IL7R transcripts from human primary CD4 + T cells transfected with control (ASO-Ctrl) or experimental (IL7R-001, IL7R-004, IL7R-005 and IL7R-006) morpholino ASOs. Percent (%) exon 6 exclusion (mean ± S.D.) is shown for each ASO under the gel image. (B) Levels of secreted sIL7R (mean ± S.D.) in supernatants from cells in panel A . (C) Relative MFI values (mean ± S.D.) of mIL7R cell surface expression in cells from panel A . (*) p < 0.05 , (**) p < 0.01 , and (***) p < 0.001 .
Techniques Used: Transfection, Control, Expressing
Figure Legend Snippet: Control (ASO-Ctrl) or lead anti-sIL7R morpholino ASOs IL7R-005 and IL7R-006 were transfected at 10 µM with Endo-Porter into HeLa cells stably expressing versions of the GFP-IL7R reporter containing either the protective ‘T’ allele or the risk ‘C’ allele of the MS SNP rs6897932. (A) Representative gel image of exon 6 splicing in transcripts from the GFP-IL7R reporter containing either the protective ‘T’ allele or the risk ‘C’ allele of the MS SNP rs6897932, and transfected with control (ASO-Ctrl) or lead anti-sIL7R (IL7R-005 and IL7R-006) morpholino ASOs. Percentage of exon 6 exclusion (mean ± S.D.) for each condition is plotted below the gel image. (B) GFP MFI (mean ± S.D.) from cells in panel A shown relative to the T reporter treated with ASO-Ctrl. (*) p < 0.05 , (**) p < 0.01 , and (***) p < 0.001 .
Techniques Used: Control, Transfection, Stable Transfection, Expressing
Figure Legend Snippet: (A) Representative gel image of exon 6 splicing in transcripts from the GFP-IL7R reporter containing either the MS risk ‘C’ allele or the protective ‘T’ allele of the IL7R SNP rs6897932, and transfected with control (ASO-Ctrl) or lead pro-sIL7R (IL7R-001 and IL7R-004) morpholino ASOs. Percentage of exon 6 exclusion (mean ± S.D.) for each condition is plotted below the gel image. (B) GFP MFI (mean ± S.D.) from cells in panel A shown relative to the C reporter treated with ASO-Ctrl. (***) p < 0.001 .
Techniques Used: Transfection, Control
Related Articles
Mutagenesis:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. 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In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Blocking Assay:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Sequencing:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Stable Transfection:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Expressing:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Transfection:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Control:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Fluorescence:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Concentration Assay:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Knockdown:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Western Blot:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Reverse Transcription Polymerase Chain Reaction:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Enzyme-linked Immunosorbent Assay:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( Transduction:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( shRNA:Article Title: Antisense modulation of IL7R splicing to control sIL7R expression in human CD4 + T cells Article Snippet: Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.Supernatants from ASO-treated HeLa cells or human primary CD4 + T cells were collected and 400 μL of each were concentrated to 100 μL by centrifugation using Amicon Ultra 3K centrifugal filters (Millipore Sigma), and the 100 μL of the concentrated supernatants were used to quantify sIL7R secretion in ELISA assays.. Measurements of secreted sIL7R in HeLa cell supernatants were conducted as follows: 96-well plate (R&D Systems) were coated at 4 ° C overnight with a Article Title: Method to identify subjects at higher risk to develop an autoimmune disease based on genetic and/or phenotypic screening for epistatic variants in DDX39B (RS2523506) and IL7R (RS6897932) Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in (Crawley et al., 2010).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4° C. overnight with a mouse anti-human IL7R monoclonal antibody ( Article Title: Human epistatic interaction controls IL7R splicing and increases Multiple Sclerosis risk Article Snippet: Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).Secreted sIL7R was quantified from the concentrated supernatants by ELISA as in ( Crawley et al., 2010 ).. In brief, 96-wells plate (R&D Systems, DY990) were coated at 4°C overnight with a mouse anti-human IL7R monoclonal antibody ( |